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KMID : 0900919920160010039
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Korean journal of Animal Reproduction
1992 Volume.16 No. 1 p.39 ~ p.46
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Fertilization of Porcine Oocytes and Culture of Embryo in Hydrogel Chambers implanted in the Peritoneal Cavity of intermediate Mouse Recipients
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Kim Myung-Cheol
Shin Sang-Tae
Park Chang-Sik
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Abstract
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In viro fertilizatin is very important in both human clinical practice and animal breeding. However, the success rate of in vitro fertilization is not high. The purpose of this study ws to determine wheter in the vitro fertilization and culture of porcine oocyte using a hydrogel chamber were possible or not. Hydrogel chambers were made of polymerized 2-hydroxyethyl methacrylate. Matured follicular oocytes in Waymouth’s medium and T L Hepes medium, tubal oocytes, and preincubated sperm in M199 medium were treansferred into the lumen of the hydrogel chambers. The chambers containing porcine oocytes and spermatozoa implanted into the mouse peritioneal cavity, and ova were examined after the recovery of the chambers at 84 hours after preservation start. The result was shown that fertilization and culture of porcine oocytes were successfully achieved inside of the hydrogel chamber.
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KEYWORD
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in vivo fertilization, in vivo culture, hydrogel chambers, porcine
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